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Histopathological examination and evaluation

Histopathological Detection and Evaluation-Histopathological Evaluation of Target Organs Injured in Experimental Animal Models: Interpretation of Characteristic Lesions in Target Organs of Model Animals before and after Intervention. Histopathological evaluation of domestic animals, companion animals, aquatic animals and wild animals.

Product Name	Category	Operation Item	Product Description
oftenOrganization pre-treatment and dyeing	Pre-tissue processing	Regular decalcification (-)	1. Scope of application: bone tissue, immunohistochemistry, HE and other staining. 2. Operation method: chelation decalcification, the cycle is 1 month and above, depending on the calcium content, protect the antigen.
		Regular decalcification (II)	1. Scope of application: bone tissue, HE staining and other special staining. The. Method of operation: rapid decalcification, the cycle is about 1 week, the antigen is easily destroyed.
		Gel embedding	1. Scope of application: cells, tiny tissues, etc. The. Methods of operation: The tissue was centrifuged to the bottom of the tube and embedded in the gel.
		Swiss Roll Embedding	1. Scope of application: gastrointestinal tissue. The operation method: will be rolled into the Swiss roll of the organization for repair block embedding processing.
		Tissue repair block embedding	1. Scope of application: All organizations The. Operation method: according to the regular operation process.
	Slice	Ordinary paraffin section	1. Scope of application: all tissues, used for HE staining and special staining. The operation method: paraffin section.
		Anti-paraffin section	1. Scope of application: all tissues, for immunohistochemistry, immunofluorescence, TUNEL. The operation method: anti-off slice.
		Frozen section	1. Scope of application: fresh tissue, used for oil red staining and immunofluorescence. The operation method: frozen section.
	HE staining	HE staining	Applicable to all organizations.
Immune tissue chemical staining and analysis	Antibody concentration groping	Immunohistochemical single staining Antibody touch concentration conditions	1. Scope of application: All organizations. Paraffin sections or ice sections of 4% paraformaldehyde. 2. Operation method: Use 2 samples and set 3 antibody concentration gradients to touch the staining concentration of anti-detachment sections and take photos, 3. Test purpose: to explore the specificity of antibody, the best antibody concentration, protein up to the site.
		Immunohistochemical double staining Antibody touch concentration conditions
		Immunofluorescence single staining Antibody touch concentration conditions
		Immunofluorescence double staining Antibody touch concentration conditions
	Immunohistochemistry and fluorescence staining	No night histochemical staining: single staining	Single staining for specific proteins, semi-quantitative, positioning.
		Free night histochemical staining: double staining	Double staining for specific proteins to achieve semi-quantitative, positioning.
		TUNEL staining	Apoptotic cells were observed to achieve semi-quantitative and localization.
		Immunofluorescence staining (1): Single dye DAPI	1. Scope of application: reflect the specific protein expression, to achieve positioning and semi-quantitative. 2. Operation method: use fluorescent antibody to directly or ask for fluorescence.
		Court-free dragon color (21: Double dye DAP
		Free Staining (3): Tri-dyed DAA
	Immunohistochemistry and TUNEL analysis	Target protein cumulative light Density/average light density Degree/Count	1. Scope of application: measurement and analysis of a target site. 2. Operation method: measure the egg white light density/average light density, count.
	Immunohistochemical double staining analysis	Target protein cumulative light Density/average light density Degree/Count
	Immunofluorescence section analysis	Immunofluorescence qualitative
		Single dye: 1 target protein DAP nuclear marker
		Double staining: 2 target proteins DAPI nuclear marker
		Three stains: 3 target proteins DAPI nuclear marker

Product Name	Operation Item	Product Description
Special dyeing	PAS staining	Applicable organization: gastrointestinal tissue, kidney, eyeball, etc,
	AB-PAS staining	Applicable organization: gastrointestinal tissue, etc.
	MASSON staining	Applicable organization: gastrointestinal, liver, heart, kidney, lung, skin, muscle, fat, reproductive system, etc.
	Safranin fast green staining (bone tissue)	Applicable organization: bone tissue, etc.
	Toluidine blue staining	Applicable organization: joint, brain, immune tissue, etc.
	Oil red staining	Applicable tissues: liver, heart, muscle, etc.
	Sirius red stain	Applicable organization: liver, heart, kidney, lung, skin, muscle, fat, etc.
	Congo red staining	Applicable organization: liver, kidney, brain, etc.
	Prussian blue staining	Applicable tissue: liver, heart containing hemosiderin or containing ferritin.